Journal: Cell Reports Medicine

Article Title: Identification and validation of intratumoral microbiome associated with sensitization to immune checkpoint inhibitors

doi: 10.1016/j.xcrm.2025.102306

Figure Lengend Snippet: Validation of selected microbes with a mouse melanoma model (A) The workflow of B16-F10 tumor-bearing mice experiment. (B) Tumor volume of mice measured every other day during the indicated treatment regimen; PBS + IgG ( n = 8), PBS + anti-PD-1 ( n = 8), P. megaterium + IgG ( n = 9), B. cepacia + IgG ( n = 9), C. kroppenstedtii + IgG ( n = 9), P. megaterium + anti-PD-1 ( n = 8), B. cepacia + anti-PD-1 ( n = 9), and C. kroppenstedtii + anti-PD-1 ( n = 6). (C) Tumor weight of mice measured at the end of the experiment; PBS + IgG ( n = 8), PBS + anti-PD-1 ( n = 7), P. megaterium + IgG ( n = 7), B. cepacia + IgG ( n = 7), C. kroppenstedtii + IgG ( n = 9), P. megaterium + anti-PD-1 ( n = 7), B. cepacia + anti-PD-1 ( n = 9), and C. kroppenstedtii + anti-PD-1 ( n = 6). (D) Flow cytometry analyses on the proportions of tumor-infiltrating CD8 + T cells, IFN-γ + CD8 + T cells, and NK1.1 + NK cells and the expression of MHC-II and PD-L1 by CD11b + F4/80 + macrophages and CD11c + DCs shown as mean fluorescent intensity. n = 6–7 per group. (E) Representative images showing immunofluorescence staining of CD8 (green), IFN-γ (red), and DAPI (blue) in tumor tissues. Scale bars, 50 μm. (F) Representative images showing immunofluorescence staining of CD8 (green) and DAPI (blue) and FISH staining of B. cepacia or P. megaterium (red) in tumor tissues. Scale bars, 100 μm. (G) Quantification of CD8 in tumor nests ( n = 16 for bacteria-enriched nests and n = 16 for bacteria-poor nests pooled from four B16F10 tumor tissues, in the P. megaterium + anti-PD-1 or B. cepacia + anti-PD-1 group). (H) Relative mRNA expression of tumor CXCL9, CXCL10, PD-L1, CCL5, TNF-α, and PRF1 from B16-F10 bearing mice. n = 4–5 per group. (I) Immune cell subsets of CibersortX from RNA-seq analysis of B16-F10 tumors. n = 4–5 per group. (J) Immune response-related signatures score from RNA-seq analysis of B16-F10 tumors. n = 4–5 per group. (K) The workflow of A375 tumor-bearing huPBMC-C-NKG mice experiment. (L) Tumor volume of mice measured every other day during the indicated treatment regimen; PBS + IgG ( n = 4), PBS + anti-PD-1 ( n = 4), and B. cepacia + anti-PD-1 ( n = 4). (M) Tumor weight of mice measured at the end of experiment; PBS + IgG ( n = 4), PBS + anti-PD-1 ( n = 4), and B. cepacia + anti-PD-1 ( n = 4). (N) Flow cytometry analyses on the proportions of tumor-infiltrating IFN-γ + CD8 + T cells. n = 4 per group. Data are shown as mean ± SEM (B–D, G–J, and L–N). Statistics are analyzed by two-way ANOVA with Tukey’s multiple comparison test (B and L), one-way ANOVA with Tukey’s multiple comparison test (C, D, H–J, M, and N), and unpaired parametric t test (G); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. See also and .

Article Snippet: Priestia megaterium , DSMZ , DSM: 32.

Techniques: Biomarker Discovery, Flow Cytometry, Expressing, Immunofluorescence, Staining, Bacteria, RNA Sequencing, Comparison

Journal: PLOS One

Article Title: Polyhydroxyalkanoate production in Priestia megaterium strains from glycerol feedstock

doi: 10.1371/journal.pone.0322838

Figure Lengend Snippet: P . megaterium strains examined in this study.

Article Snippet: DSM319 , DSMZ , CP001982 , .

Techniques: